ABSTRACT
Objective:To simultaneously separate 5 phenylpropanoids from Cinnamomum cassia by semi-preparative HPLC, and explore their immunosuppressive activity. Methods:After extracted by ethanol, the ethyl acetate part of Cinnamomum cassia was isola-ted by semi-preparative HPLC. The separation was conducted on an Ultimate XB-C18 (250 mm × 10 mm, 5μm) semi-preparative chro-matographic column and the mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 330 nm, and the flow rate was 2. 5 ml·min-1 . The sample volume was 0. 3 ml. MS and NMR spectroscopic analysis were used to determine the config-urations. A CCK-8 method was used to detect the immunosuppressive activity of phenylpropanoids. Results: Totally 5 phenylpro-panoids were separated from Cinnamomum cassia by the semi-preparative HPLC, and identified as erythro-guaiacylglycerol, (7R,8S)-syringoylglycerol, (7S,8S)-syringoylglycerol, 3-methoxyphenyl-acrylaldehyde and O-methoxy cinnamaldehyde. The inhibitory rates of T cells and B cells of the compound 3 was more than 20% at the concentration of 800μmol·L-1 . Conclusion:The method is conven-ient with good separation effect, which can simultaneously separate 5 phenylpropanoids from Cinnamomum cassia, and among them, the compound 3 shows immunosuppressive effect to some extent.
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To investigate the anti-inflammatory, analgesia and antipyretic effects of Chaige Qingre granules. Meth-ods:Kunming mice were used to make auricle swelling model by dimthylphenanthrene, and the auricle swelling degree was measured to test the anti-inflammatory effect of Chaige Qingre granules. Mice were used to make pain models by acetic acid, and the body twis-ting of the mice was counted to observe the analgesia effect of the granules. The fever model in rats was induced by yeast, and the body temperature of the rats was measured to investigate the antipyretic effects of the granules. Results:Chaige Qingre granules could relieve the auricle swelling degree significantly, and the inhibition rate was 46. 3% in the high dose group. High and middle dose groups could reduce the body twisting frequency with the inhibition rate of 51. 1% and 61. 8%, respectively. Every Chaige Qingre granules group could lower the body temperature after one hour of the administration, and the temperature could be reduced 0. 56 degrees Celsius in the high dose group. Conclusion:Chaige Qingre granules can effectively inhibit auricle swelling degree, relieve pain and reduce heat.
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Objective To investigate the mid-term effect of SolitaireAB stent-assisted interventional embolization with spring coils for the treatment of intracranial wide-necked aneurysms. Methods During the period from May 2009 to April 2013, a total of 49 patients with intracranial wide-necked aneurysm (49 aneurysms in total) received SolitaireAB stent-assisted interventional embolization treatment at authors’ hospital. In 41 patients, a total of 41 aneurysms were detected, of which ruptured aneurysm with bleeding was confirmed in 26 and un-ruptured aneurysm in 15. These 41 patients were followed up for 12-48 months. Based on modified Rankin scoring and DSA, CTA or MRA manifestations, the clinical results were evaluated. Results After the embolization treatment, re-bleeding of the aneurysm occurred in 2 cases, cerebral infarction in 3 cases, occlusion of the parent artery in one case and death in one case; the occurrence rate of complications was 14.2%. DSA, MRA or CTA performed at 12 months after the embolization treatment, showed that 32 aneurysms (78.0%) were completely obstructed, which was obviously higher than that observed on DSA performed immediately after the embolization procedure (21 aneurysms, 42.9%), the difference was statistically significant (P=0.02);residue of aneurismal neck was seen in 7 cases (17.1%) and partial occlusion in 2 cases (4.9%), which were much better than those observed on DSA that was performed immediately after the embolization procedure. Twenty-four aneurysms (58.5%) remained stable, showing no any change, and recurrence of aneurysm was observed in 4 cases (9.7%). At the last follow-up exam, the modified Rankin scoring showed that 0 point was seen in 18 cases (43.9%), one point in 10 cases (24.4%), 2 points in 5 cases (12.2%), 3 points in 4 cases (9.8%), 4 points in 2 cases (4.85%) and 5 points in 2 cases (4.85%). The self-care rate for daily activities was 80.5%, the prognosis was good. Conclusion For the treatment of intracranial wide-necked aneurysms, SolitaireAB stent-assisted interventional embolization with spring coils is safe and effective. This technique can improve the embolization rate and reduce the procedure-related complications.
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OBJECTIVE:To study the pharmacokinetics and serum fingerprints of Decoction for Puerperal Blood Stasis at different time.METHODS:The content of Ferulaic acid(FA) was determined by HPLC.The pharmacokinetic study was carried out.The active constituent in the Decoction for Puerperal Blood Stasis was analyzed based on the serum fingerprints of FA.RESULTS:The distribution and metabolism of FA in rabbits after oral administration of Decoction for Puerperal Blood Stasis assummed two compartment model.The fingerprints between serum at different time and blank serum showed significant differences.CONCLUSION:The content change of FA monitored with serum fingerprints was in line with the rule of pharmacokinetics of FA in Decoction for Puerperal Blood Stasis.The fingerprints can be applied to clarify the pharmacokinetic characteristics,the pharmacological action and the action mechanism of Decoction for Puerperal Blood Stasis.